detector

Animation of chromatographic separation

Model of the separation, during a chromatography, of 2 types of molecules (coloured circles) with different retention on the chromatographic matrix (also, stationary phase or column packing).

Note how in addition to getting separated they disperse while progressing through the column, leading to band widening.

Press to initiate or repeat the animation. 

Speed:

Chromatogram:
concentration
elution time or volume