Nonlinear regression for Michaelis & Menten kinetics
Please specify the
format
of your data:
one [S], one ѵ
one [S], several ѵ (replicates)
one [S], one ѵ, standard error of ѵ
Check
if they use decimal comma.
State the units you are using for your data:
[S] in
mM
µM
ѵ
in
µM/min
µM/s
Please type (or paste) the
[S]
,
ѵ
data
:
0.5, 9 1, 23 2, 32 5, 36
clear all data
Either accept these initial
estimated values
, or enter others:
ѵ
max
=
(
±
)
p=
K
m
=
(
±
)
p=
RMS =
One more calculation step
v
[S]
Press the button. Observe above how the parameters and the RMS change. If the new values are reasonable, press the button more times until the improvement indicators change to green.
If the parameters diverge, try with new initial estimates.
1.0
0.5
0.2
0.1
0.05